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Digital Spotlight Series

The BioTechniques Digital Spotlight Series is a collection of news and peer-reviewed content focused on a revolving series of in-demand topics selected by the editors of BioTechniques. Each issue feautres news and peer-reviewed content selected by the editors of BioTechniques

The Digital Spotlight Series will publish on a new BioTechniques digital platform that features:

  • Live-linking
  • Social sharing
  • Notes and bookmarking – Readers can highlight ads, article text, create sticky notes and bookmark pages for future reference

Advertising Packages:

  • Bronze: Digital Edition delivered to targeted audience with 3 full page advertisements. Single-sponsorship.
  • Silver: Digital Edition delivered to specified targeted audience with 3 full page advertisements and an application note. Single-sponsorship.
  • Gold: Digital Edition Delivered to specified targeted audience with 3 advertisements, an application note, and a sponsored video clip. Single-sponsorship.

View previous issues of the BioTechniques Digital Spotlight series and monthly journal issues online: http://www.BioTechniques.com/Digital

Publishing Schedule: (Reach)

  • Cell Culture: Available (45,100)
  • Microscopy/Fluorescent Probes: Available (45,900)
  • Next-Generation Sequencing: Available (87,424)
  • Stem Cells: Available (46,194)
  • Micro RNAs: Available (70,317)
  • PCR: Published
  • Imaging: Published
  • Transfection: Published

Editorial Details:

Spotlight On Cell Culture

Articles Include:

  • Introduction from the Editor-in-Chief: The Many Dimensions of Cell Culture
  • Cell Culture in the News: “Stem Cell Factors differ in Mice and Men” and “Shape-shifting cell cultures”
  • Troubleshooting forum on challenges of effective cell culture
  • Peer-reviewed articles on Cell Culture Applications:
    • Zeocin for selection of bleMX6 resistance in fission yeast
    • The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without
      interference from background fluorescence
  • Peer-reviewed articles on Cell Culture Techniques:
    • Small RNA fragments in complex culture media cause alterations in protein profiles of three species of
      bacteria
    • Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D
      cultures

Spotlight On Microscopy / Fluorescent probes

Articles Include:

  • Introduction from the Editor-in-Chief: Imaging the rainbow
  • Imaging Technology in the News: “Turbo boost for confocal microscopy” and “Brain STORMing”
  • Troubleshooting Forum on microscopy and applications
  • Peer-reviewed articles on probes and dyes in mircroscopy:
    • Modern fluorescent proteins: from chromophore formation to novel intracellular applications
    • YGFP: a spectral variant of GFP
  • Peer-reviewed articles on imaging surfaces and depth:
    • Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy
    • Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopy
  • Peer-reviewed articles on High-throughput imaging:
    • Automated feature detection and imaging for high-resolution screening of zebrafish embryos

Spotlight On Next generation sequencing

Articles Include:

  • Introduction from the Editor-in-Chief: The growing application base for NGS
  • NGS in the News: From DVD to DNA: “Next-generation DNA data storage” and “The promise and pitfalls of thirdgeneration
    sequencing”
  • Troubleshooting Forum on NGS techniques and applications
  • Peer-reviewed articles on:
    • Direct sequencing of small genomes on the Pacific BioSciences RS without library preparation
    • cDNA normalization by hydroxyapatite chromatography to enrich transcriptome diversity in RNA-seq
      applications
    • Creation of immortalized bait libraries for targeted enrichment and next-generation sequencing
    • Short-read, high-throughput sequencing technology for STR typing
    • Length and GC-biases during sequencing library amplification: a comparison of various polymerase-buffer
      systems with ancient and modern DNA sequencing libraries

Spotlight On Stem Cells

Articles Include:

  • Introduction from the Editor-in-Chief: Expanding the stem cell niche
  • Cell Culture in the News: “Cancer stem cells fuel tumor growth in mice guts” and “Fine-tuning stem cell imaging”
  • Troubleshooting forum on stem cells
  • Peer-reviewed articles on:
    • Induced G1 phase arrest of fast dividing cells improves the quality of genomic profiles generated by array-
      CGH
    • High-throughput methods to define complex stem cell niches
    • Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy
    • Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis
    • Emerging molecular approaches in stem cell biology

Spotlight On MicroRNAs

Articles Include:

  • Introduction from the Editor-in-Chief: The expansion of miRNAs
  • Imaging Technology in the News: “RNAi Therapeutics: The Teenage Years, New Song, New miRNA levels”, and
    “mRNA methylation widespread and likely functional”
  • Troubleshooting Forum on new miRNA tools
  • Peer-reviewed articles on probes and dyes in microscopy:
    • Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization
    • In situ detection of mature microRNAs by labeled extension on ultramer templates
    • Concordance among digital gene expression, microarrays, and qPCR when measuring differential expression
      of microRNAs
    • Protein-mediated miRNA detection and siRNA enrichment using p19
    • Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep
      sequencing

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