Digital Spotlight Series
The BioTechniques Digital Spotlight Series is a monthly collection of news and peer-reviewed content focused on a revolving series of in-demand topics selected by the editors of BioTechniques.
The Digital Spotlight Series will publish on the all new BioTechniques digital platform that features:
- Live-linking
- Social sharing
- Notes and bookmarking – Readers can highlight ads, article text, create sticky notes and bookmark pages for future reference
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View previous issues of the BioTechniques Digital Spotlight series and monthly journal issues online: http://www.BioTechniques.com/Digital
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Publishing Schedule:
- January: PCR
- February: Cell Culture
- March: Microscopy/Fluorescent probes
- April: Next-generation Sequencing
- May: Stem Cells
- June: MicroRNAs
Editorial Details:
January – Spotlight on PCR
Articles Include:
- Introduction from the Editor-in-Chief: Recent Advance and Applications of PCR
- PCR in the News: “Synthetic Bio: Expanding PCR’s Repertoire” and “How Reliable is Real-time PCR?”
- Troubleshooting forum on PCR optimization
- Peer-reviewed articles on qPCR:
- Simultaneous multiple target detection in real-time loop-mediated isothermal amplification
- A qPCR-based assay to quantify oxidized guanine and other FPG-sensitive base lesions within telomeric DNA
- The use of melting curves as a novel approach for validation of real-time PCR instruments
- Peer-reviewed articles on applications in design for PCR:
- Construction of effective inverted repeat silencing constructs using sodium bisulfite treatment coupled with
strand-specific PCR - Computation-assisted SiteFinding- PCR for isolating flanking sequence tags in rice
- Construction of effective inverted repeat silencing constructs using sodium bisulfite treatment coupled with
February – Spotlight On Cell Culture
Articles Include:
- Introduction from the Editor-in-Chief: The Many Dimensions of Cell Culture
- Cell Culture in the News: “Stem Cell Factors differ in Mice and Men” and “Shape-shifting cell cultures”
- Troubleshooting forum on challenges of effective cell culture
- Peer-reviewed articles on Cell Culture Applications:
- Zeocin for selection of bleMX6 resistance in fission yeast
- The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without
interference from background fluorescence
- Peer-reviewed articles on Cell Culture Techniques:
- Small RNA fragments in complex culture media cause alterations in protein profiles of three species of
bacteria - Planar arrangement of eukaryotic cells in merged hydrogels combines the advantages of 3-D and 2-D
cultures
- Small RNA fragments in complex culture media cause alterations in protein profiles of three species of
March – Spotlight On Microscopy / Fluorescent probes
Articles Include:
- Introduction from the Editor-in-Chief: Imaging the rainbow
- Imaging Technology in the News: “Turbo boost for confocal microscopy” and “Brain STORMing”
- Troubleshooting Forum on microscopy and applications
- Peer-reviewed articles on probes and dyes in mircroscopy:
- Modern fluorescent proteins: from chromophore formation to novel intracellular applications
- YGFP: a spectral variant of GFP
- Peer-reviewed articles on imaging surfaces and depth:
- Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy
- Thickness profiling of formaldehyde-fixed cells by transmission-through-dye microscopy
- Peer-reviewed articles on High-throughput imaging:
- Automated feature detection and imaging for high-resolution screening of zebrafish embryos
April – Spotlight On Next generation sequencing
Articles Include:
- Introduction from the Editor-in-Chief: The growing application base for NGS
- NGS in the News: From DVD to DNA: “Next-generation DNA data storage” and “The promise and pitfalls of thirdgeneration
sequencing” - Troubleshooting Forum on NGS techniques and applications
- Peer-reviewed articles on:
- Direct sequencing of small genomes on the Pacific BioSciences RS without library preparation
- cDNA normalization by hydroxyapatite chromatography to enrich transcriptome diversity in RNA-seq
applications - Creation of immortalized bait libraries for targeted enrichment and next-generation sequencing
- Short-read, high-throughput sequencing technology for STR typing
- Length and GC-biases during sequencing library amplification: a comparison of various polymerase-buffer
systems with ancient and modern DNA sequencing libraries
May – Spotlight On Stem Cells
Articles Include:
- Introduction from the Editor-in-Chief: Expanding the stem cell niche
- Cell Culture in the News: “Cancer stem cells fuel tumor growth in mice guts” and “Fine-tuning stem cell imaging”
- Troubleshooting forum on stem cells
- Peer-reviewed articles on:
- Induced G1 phase arrest of fast dividing cells improves the quality of genomic profiles generated by array-
CGH - High-throughput methods to define complex stem cell niches
- Label-free detection of surface markers on stem cells by oblique-incidence reflectivity difference microscopy
- Isolation of Sertoli, Leydig, and spermatogenic cells from the mouse testis
- Emerging molecular approaches in stem cell biology
- Induced G1 phase arrest of fast dividing cells improves the quality of genomic profiles generated by array-
June – Spotlight On MicroRNAs
Articles Include:
- Introduction from the Editor-in-Chief: The expansion of miRNAs
- Imaging Technology in the News: “RNAi Therapeutics: The Teenage Years, New Song, New miRNA levels”, and
“mRNA methylation widespread and likely functional” - Troubleshooting Forum on new miRNA tools
- Peer-reviewed articles on probes and dyes in microscopy:
- Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization
- In situ detection of mature microRNAs by labeled extension on ultramer templates
- Concordance among digital gene expression, microarrays, and qPCR when measuring differential expression
of microRNAs - Protein-mediated miRNA detection and siRNA enrichment using p19
- Reduction of non-insert sequence reads by dimer eliminator LNA oligonucleotide for small RNA deep
sequencing