Editorial Focus

Editorial Overview

BioTechniques brings readers news features and peer-reviewed research articles focusing on the latest in methods and techniques for lab scientists. From informative Tech News features to primary research articles that can have an immediate impact in the lab, each issue has something for everyone.

In 2014, BioTechniques will publish four special Focus Sections celebrating 30 Years of BioTechniques:

  • A Practical Guide to DNA Sequencing – From library preparation to data analysis, this special section will include articles aimed at providing practical advice for novice, and experienced, scientists working with next-generation sequencing instruments and kits.
  • A Practical Guide to qPCR – PCR revolutionized molecular biology. qPCR has expanded researchers’ abilities to quantify samples and biomolecules. Still, additional information is needed to obtain reliable qPCR results. In this section, articles focusing on qPCR approaches and data normalization techniques will be presented.
  • A Practical Guide to Cell Culture – Culturing cells is a fundamental technique in the modern biology lab. Here, we provide insights into best cell culture practices and approaches to enhance culture conditions and experimental outcomes.
  • A Practical Guide to Super-resolution Microscopy – This focus section will explore approaches to using super-resolution microscopy. From STED to PALM and STORM, this practical guide is intended to help researchers get the most out of these emerging technologies.

In 2015, BioTechniques will publish six Practical Guide features including:

PCR 2015 – From qPCR to digital PCR and multiplex PCR, this first Practical Guide for 2015 examines different PCR applications, providing practical guidance for best experimental results.

Genome Engineering – Genome engineering is becoming commonplace with the development of tools such as ZFNs, TALENs, and CRISPR-Cas9. This Practical Guide explores the latest techniques and protocols for modifying genes and DNA segments in a variety of organisms.

Protein Interactions – FRET can be used to assay distances between biomolecules as well as examine folding kinetics inside single proteins. This Practical Guide investigates the many uses of FRET and provides guidance for implementing in any lab.

Genome Structure and Function – This Practical Guide examines the latest methods and techniques used to study how genomes are organized and gene expression is regulated.

What do I do now that I have my NGS data? It is easy to generate volumes of sequence data, but what do you do with all of that information? What are the best data/ sequence analysis tools? This Practical Guide will assist readers in finding the proper bioinformatics tools to get the most out of their sequencing experiments.

Stem Cells – Paths to Pluripotency – This Practical Guide will detail various methods now available to generate, culture and maintain pluripotent stem cells. From genetic manipulation to chemical transformation, protocols will be examined and case studies presented.

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